Try: BRCA1 TP53 EGFR APOE CFTR ELFN1 | Contact

Auto SMART Editing Design

Automate SMART editing design for CRISPR/Cas9-mediated insertion, deletion, and point mutations via the homology-directed repair pathway

Try: BRCA1 TP53 EGFR APOE CFTR ELFN1
Zhao, C., Cao, Y., Ibrahim, N. et al. Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization. Nat Commun 16, 3768 (2025). https://doi.org/10.1038/s41467-025-58945-6
Auto-SMART is free for academic and non-profit research use. SMART editing is covered by patent WO2026039431; for commercial use, please refer to the patent.
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() Genome Transcript CHR Strand bp
SEQUENCE MAP
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Click & drag DNA to select for CRISPR

Gene Overview — · drag to select region

Sequence Map

EDITING OPERATIONS

DESIGN CRISPR

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SpCas9 · NGG PAM · 20nt guide · On-target: Doench 2016 (Rule Set 2, human-trained) · Off-target: CRISPOR
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Start an edit

Click & drag across bases in the sequence map, or drag on the Gene Overview above, to select a region — then pick an operation: insertion, deletion, point mutation, replacement, or gRNA design.