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Auto SMART Editing Design

Automate SMART editing design for CRISPR/Cas9-mediated insertion, deletion, and point mutations via the homology-directed repair pathway

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() Genome Transcript CHR Strand bp
SEQUENCE MAP
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Click & drag DNA to select for CRISPR

Gene Overview — · drag to select region

Sequence Map

EDITING OPERATIONS

➕
Insertion
Insert bases between two selected nucleotides
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Deletion
Delete selected nucleotides
⇄
Point mutations
Mutate individual nucleotides
↻
Sequence Replacement
Replace a range with a new sequence via SMART HDR
✂
Design gRNAs
Find CRISPR guide RNAs in selected region

DESIGN CRISPR

– ( bp)
SpCas9 · NGG PAM · 20nt guide · On-target: Doench 2016 (Rule Set 2, human-trained) · Off-target: CRISPOR